Page 189 has the base rules for Fatigue. I'd say running a Locust all over the desert for hours on end would probably inflict at least 1 point of Fatigue per hour. You could also inflict a few points for less than six hours of sleep. In my experience, 90 minutes is a sleep cycle, and I need at least three of those to be functional. Four is better (six hours of sleep). Some research says most people need 8 hours.